Proline is the second most abundant amino acid in Aphasol and an amino acid that plays a crucial role in helping plants cope with cold stress. Cold stress during early spring in fruit orchards that can cause damage to blossoms, buds and during cold winter circumstances on the leaves of leek and winter cauliflower and disrupt normal metabolic processes and disrupt normal metabolic processes.

Under cold stress conditions, proline acts as an osmoprotectant, helping the plant to maintain its cellular hydration and preventing damage from ice crystal formation. Proline helps to stabilise plant cells by reducing the movement of water molecules from the plant cells towards ice crystals, and as such protects the cells from dehydration. This helps to prevent damage to the cell membrane and other cellular components.

In addition to its role as an osmoprotectant, proline also plays a role in regulating plant metabolism during cold stress. It has been shown to regulate the expression of stress-responsive genes and increase the production of antioxidants, which helps to protect the plant from cold-induced oxidative stress.

Overall, the role of proline in plants suffering from cold stress is to help maintain cellular stability, regulate plant metabolism, and protect the plant from cold-induced damage.